This study explored the antimicrobial activity of the antioxidant phenolic compounds in lingonberry juice and two other fruits in spoiled fruit juice. They studied Asaia lannensis and Asaia bogorensis, two well known bacteria that are a significant contributor to the degradation of non-alcoholic fruit juices. These bacteria create biofilms that cause turbidity and adhesion of the juice on surfaces holding the juice. These biofilms, in turn, can cause illness in susceptible individuals. The bacteria are also becoming resistant to a lot of the chemical preservatives used now in juices. The authors found that lingonberry juice added to the product shows a 67% reduction in adhesions from the bacteria. We all knew lingonberries were great. The evidence keeps mounting!
Wild Fruits as Antiadhesive Agents Against the Beverage-Spoiling Bacteria Asaia spp.
Hubert Antolak, Agata Czyzowska , Marijana Saka , Aleksandra Mišan , Olivera uragi´c and Dorotea Kregiel Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland; firstname.lastname@example.org (A.C.); and Institute of Food Technology Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia;
Abstract: The aim of the study was to evaluate antioxidant activity and total phenolic content of juice from three different types of fruits: elderberry (Sambucus nigra), lingonberry (Vaccinium vitis-idaea) and cornelian cherry (Cornus mas), and their action against adhesion of bacterial strains of Asaia lannensis and Asaia bogorensis isolated from spoiled soft drinks. The antioxidant profiles were determined by total antioxidant capacity (2,2-diphenyl-1-picrylhydrazyl, DPPH), and ferric-reducing antioxidant power (FRAP). Additionally, total polyphenol content (TPC) was investigated. Chemical compositions of juices were tested using the chromatographic techniques: high-performance liquid chromatography (HPLC) and liquid chromatography–mass spectrometry (LC-MS). Adhesion properties of Asaia spp. cells to various abiotic materials were evaluated by luminometry, plate count and fluorescence microscopy. Antioxidant activity of fruit juices expressed as inhibitory concentration (IC50) ranged from 0.042 0.001 (cornelian cherry) to 0.021 0.001 g/mL (elderberry). TPC ranged from 8.02 0.027 (elderberry) to 2.33 0.013 mg/mL (cornelian cherry). Cyanidin-3-sambubioside-5-glucoside, cyanidin-3-glucoside, and cyanidin-3-sambubioside were detected as the major anthocyanins and caffeic, cinnamic, gallic, protocatechuic, and p-coumaric acids as the major phenolic acids. A significant linear correlation was noted between TPC and antioxidant capacity. In the presence of fruit juices a significant decrease of bacterial adhesion from 74% (elderberry) to 67% (lingonberry) was observed. The high phenolic content indicated that these content indicated that these compounds may contribute to the reduction of Asaia spp. adhesion.